Human Phb (Prohibitin) Elisa Kit

We are engaged in offering excellent quality Human PHB (Prohibitin) ELISA Kit to our valuable clients.
Detection Range : 0.313-20ng/mL
Sensitivity : 0.188 ng/mL

This kit recognizes natural and recombinant Human PHB. No significant cross-reactivity or interference between Human PHB and analogues was observed. Notei Limited by existing techniques, cross-reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human PHB and all the analogues.

Repeatability : Coefficient of variation were<10%.

Test principle:-
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PHB.Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm A 2 nm. The OD value is proportional to the concentration of Human PHB.