Product Overview
Key Features
Intended use
This ELISA kit applies to the in vitro quantitative determination of Rat C5 concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm A 2 nm. The OD value is proportional to the concentration of Rat C5. You can calculate the concentration of Rat C5 in the samples by comparing the OD of the samples to the standard curve.
Assay type:Sandwich
Format:96T
Assay time:4.5h
Reactivity:Rat
Detection Method:Colormetric
Detection Range:0.31a 20 ng/mL
Sensitivity:0.19 ng/mL
Sample Volume Required Per Well:100 I L
Company Details
Business Type
Importer, Distributor, Supplier, Trading Company
Employee Count
9
Establishment
2012
Working Days
Monday To Sunday
GST NO
07BXSPD1989H1ZY
Seller Details
SR GROUP
GST
07BXSPD1989H1ZY
Rating
4
Proprietor
Mr Rambhajan Dwivedi
Member Since
8 Years
Address
Plot No- D17/63, Rohini Sector 3, Near Ram Lila Park, Delhi, Delhi, 110085, India
elisa kits in DelhiReport incorrect details
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