Ribonuclease A (Rnase A)
Product Overview
Key Features
Appearance: White or pain white lyophilized powder
Package: 100mg/Vial
Stocking buffer
This product is mainly used to hydrolyze and remove RNA during DNA preparation:
(1) Micro-extraction of plasmid: RNase A was added to the restriction enzyme digestion reaction solution, and the final concentration was 20 I g/mLa
(2) Large scale extraction of plasmid: RNase A is added to the cell lysate, with the final concentration of 20 I g/mL, 37a , 20~60 min, then extract with phenol chloroform, and finally precipitate with 2M ammonium acetate and twice the volume of 100% ethanol.
[Note]: The addition of EDTA can inhibit the possible DNase activity.
Unite definition
At 25a , 100 mM Tris acetate (pH 6.5), 1 mM EDTA and 1 mM cyclic 2', 3'-CMP reaction solution, the amount of enzyme that increases A286 per milliliter per minute by 0.1 is defined as an enzyme activity unit (Kunitz unit).
Stability: Stable at -20a for 36 months.
Company Details
Business Type
Exporter, Manufacturer, Distributor, Supplier, Trading Company
Employee Count
100
Establishment
2009
Working Days
Monday To Sunday
Seller Details
HANGZHOU JUNFENG BIOENGINEERING CO. LTD
Export Manager
Mr. Guoan Liu
Member Since
3 Years
Address
Liuxia Industry Park 11, North Liuhe Road, Xihu District, Hangzhou, Zhejiang, 310022, China
enzymes in HangzhouReport incorrect details
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