Rnase (Ribonuclease) Inhibitor

RNase (Ribonuclease) Inhibitor is a kind of porcine liver gene RNase inhibitor expressed in recombinant strain of E. coli. It is purified by affinity chromatography to high homogeneity. This inhibitor has a very high binding affinity for pancreatic-type ribonucleases such as RNase A. Like other inhibitors of pancreatic ribonuclease, it is an acidic protein. This recombinant RNase inhibitor has a molecular weight about 67,000 daltons. It forms a 1:1 complex with bovine pancreatic RNase A, and it is a noncompetitive inhibitor of these pancreatic enzymes and protect RNA from hydrolyzing by contamitent RNase. The RNase Inhibitor is active against RNase A, RNase B, and RNase C. The Ki value is 4x10-14M. The RNase Inhibitor will inhibite RNase at pH 5-8 and maintain its inhibition at at least 1mM DTTa It does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
Storage Buffer
20 mM Tris-HCl (pH 8), 50 mM KCl, 0.5 mM EDTA, 8 mM DTT, and 50% (v/v) glycerol
Specifications
M. W.i Approximately 67 kDa.
Quantityi 40U/I l
Purityi > 95 % by SDS-PAGE
PIi 4.92
Usage
1a cDNA Synthesis When added to the reaction mixtur, it will protect mRNA and improves total cDNA yields including percent total full length of cDNA(40U/20ul).
2a RT-PCR It is compatible with all enzymes used in RT-PCR(40U/20ul).
3a In Vitro Transcription It is useful for the isolation of intact RNA transcripts using T3, T7, and SP6 RNA Polymerases(40U/0ul).
Stability
Stable at -20a for more than three years since report.