Q. What is the principle of an autoclave machine?

Answer

Using an autoclave to perform a steam sterilization involves placing the item to be sterilized in direct contact with steam at the appropriate temperature and pressure for the allotted amount of time. Thus, steam, pressure, temperature, and time are the four variables in steam sterilization. Moist heat, obtained by heating the materials inside the device over the boiling point of water, is effective at killing all microorganisms, including heat-resistant endospores. Autoclaves are often used in hospitals and research facilities to disinfect instruments and dispose of unwanted materials. Sterilization with an autoclave relies on high temperatures to destroy germs including bacteria and mold spores. Pressurized steam is used to provide the heat.